CHARACTERIZATION, CLONING AND SEQUENCING OF A THERMOSTABLE ENDO-(1,3-1,4) BETA-GLUCANASE-ENCODING GENE FROM AN ALKALOPHILIC BACILLUS-BREVIS

被引：67
|
作者：
LOUW, ME
REID, SJ
WATSON, TG
机构：
[1] CSIR,DIV FOOD SCI & TECHNOL,BIOTECHNOL PROGRAMME,POB 395,PRETORIA 0001,SOUTH AFRICA
[2] UNIV CAPE TOWN,DEPT MICROBIOL,RONDEBOSCH 7700,SOUTH AFRICA
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 1993年 / 38卷 / 04期
关键词：
D O I：
10.1007/BF00242946
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A Bacillus brevis gene coding for an endo-(1,3-1,4)-beta-glucanase was cloned in Escherichia coli and sequenced. The open reading frame contains a sequence of 759 nucleotides encoding a polypeptide of 252 amino acid residues. The amino acid sequence of the beta-glucanase gene showed only a 50% similarity to previously published data for Bacillus endo-(1,3-1,4)-beta-glucanases. The optimum temperature and pH for enzyme activity were 65-70-degrees-C and 8-10, respectively. When held at 75-degrees-C for 1 h, 75% residual activity was measured. The molecular mass was estimated to be about 29 kDa on sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis and the enzyme was found to be resistant to SDS.
引用
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页码：507 / 513
页数：7
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