TIME-RESOLVED FLUOROMETRY - PRINCIPLES AND APPLICATIONS IN CLINICAL-CHEMISTRY

被引：0
|
作者：
GAILLARD, O
KAPEL, N
GALLI, J
DELATTRE, J
MEILLET, D
机构：
[1] HOP LA PITIE SALPETRIERE, INSERM, U134, NEUROIMMUNOL LAB, PARIS 13, FRANCE
[2] HOP LA PITIE SALPETRIERE, BIOCHIM LAB, PARIS 13, FRANCE
[3] HOP BICHAT CLAUDE BERNARD, PARASITOL ORIENTAT COPROL LAB, F-75005 PARIS, FRANCE
[4] FAC MED & PHARM BESANCON, PARASITOL & MYCOL LAB, F-25030 BESANCON, FRANCE
来源：
ANNALES DE BIOLOGIE CLINIQUE | 1994年 / 52卷 / 11期
关键词：
TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY; EUROPIUM;
D O I：
暂无
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Time-resolved fluorometric assay is based on lanthanide fluorescence. This time-resolved fluorescence has a narrow-band emission line whose wavelength differs from that of emission-pulsed light and has a long decay-time. These characteristics make it possible to avoid background interference from sample constituents (protein, light-scattering particles, etc). Europium and its chelates are the most commonly used lanthanides. The europium-labeling of antigens or antibodies is followed by immunoassay. In the final step, fluorescence is measured, after enhancement, as counts per second. This assay has several advantages, including a wide working range, high sensitivity and good practicability. The method has widespread applications in the field of immunoassays in both clinical and research laboratories. The use of non-radioactive europium-labelled probes and the development of simultaneous multiple tests are possible future orientations.
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页码：751 / 755
页数：5
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