HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY GEL-ELECTROPHORESIS AS APPLIED TO ANTISENSE DNA

被引：0
|
作者：
COHEN, AS
VILENCHIK, M
DUDLEY, JL
GEMBORYS, MW
BOURQUE, AJ
机构：
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 638卷 / 02期
关键词：
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Reversed-phase and ion-exchange HPLC are compared with both slab gel and capillary gel electrophoresis tor the separation of antisense phosphorothioate oligomers. The chromatographic separations were found to be markedly inferior to the electrophoretic separations, especially for oligomers greater than 20 bases in length. The potential of gel high-performance capillary electrophoresis for the analysis of phosphorothioate analogues is shown.
引用
收藏
页码：293 / 301
页数：9
相关论文
共 50 条
  • [21] HIGH-PERFORMANCE ELECTROPHORESIS - THE ELECTROPHORETIC COUNTERPART OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    HJERTEN, S
    [J]. JOURNAL OF CHROMATOGRAPHY, 1983, 270 (NOV): : 1 - 6
  • [22] DIRECT COUPLING OF CAPILLARY LIQUID-CHROMATOGRAPHY WITH CONVENTIONAL HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    TAKEUCHI, T
    ASAI, M
    HARAGUCHI, H
    ISHII, D
    [J]. JOURNAL OF CHROMATOGRAPHY, 1990, 499 : 549 - 556
  • [23] RAPID SEPARATION AND PURIFICATION OF OLIGONUCLEOTIDES BY HIGH-PERFORMANCE CAPILLARY GEL-ELECTROPHORESIS
    COHEN, AS
    NAJARIAN, DR
    PAULUS, A
    GUTTMAN, A
    SMITH, JA
    KARGER, BL
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (24) : 9660 - 9663
  • [24] COMPARISON OF CAPILLARY ZONE ELECTROPHORESIS WITH HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY FOR THE DETERMINATION OF ADDITIVES IN FOODSTUFFS
    JIMIDAR, M
    HAMOIR, TP
    FORIERS, A
    MASSART, DL
    [J]. JOURNAL OF CHROMATOGRAPHY, 1993, 636 (01): : 179 - 186
  • [25] SEPARATIONS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ZONE ELECTROPHORESIS - A COMPARATIVE-STUDY
    ISSAQ, HJ
    JANINI, GM
    ATAMNA, IZ
    MUSCHIK, GM
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1991, 14 (05): : 817 - 845
  • [26] DETERMINATION OF CAFFEINE AND ITS METABOLITES IN URINE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS
    RODOPOULOS, N
    NORMAN, A
    [J]. SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION, 1994, 54 (04): : 305 - 315
  • [27] COMPARISON OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND CAPILLARY ELECTROPHORESIS IN THE ANALYSIS OF SOMATOSTATIN ANALOG PEPTIDES
    IDEI, M
    MEZO, I
    VADASZ, Z
    HORVATH, A
    TEPLAN, I
    KERI, G
    [J]. JOURNAL OF CHROMATOGRAPHY, 1993, 648 (01): : 251 - 256
  • [28] DISCRIMINATION OF RICE CULTIVARS BY POLYACRYLAMIDE-GEL ELECTROPHORESIS AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    HUSSAIN, A
    SCANLON, MG
    JULIANO, BO
    BUSHUK, W
    [J]. CEREAL CHEMISTRY, 1989, 66 (04) : 353 - 356
  • [29] SIZE-EXCLUSION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS OF PROTEINS - A COMPARISON
    JOSIC, D
    BAUMANN, H
    REUTTER, W
    [J]. ANALYTICAL BIOCHEMISTRY, 1984, 142 (02) : 473 - 479
  • [30] ANALYSIS OF AQUEOUS-HUMOR IN UVEITIS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY AND SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL-ELECTROPHORESIS
    MURRAY, PI
    HOEKZEMA, R
    LUYENDIJK, L
    KIJLSTRA, A
    [J]. INTERNATIONAL OPHTHALMOLOGY, 1992, 16 (01) : 15 - 22
12345