IMPROVED PURIFICATION OF 12-LIPOXYGENASE FROM RAT BASOPHILIC LEUKEMIA-CELLS AND CONDITIONS FOR OPTIMAL ENZYME-ACTIVITY

被引：5
|
作者：
VANDERDONK, EMM [1 ]
DUBOIS, GR [1 ]
VERHAGEN, J [1 ]
VELDINK, GA [1 ]
VLIEGENTHART, JFG [1 ]
机构：
[1] STATE UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT BIOORGAN CHEM,PADUALAAN 8,3584 CH UTRECHT,NETHERLANDS
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1991年 / 1074卷 / 03期
关键词：
12-LIPOXYGENASE; 13-HYDROXYOCTADECADIENOIC ACID; (RAT BASOPHILIC LEUKEMIA CELL);
D O I：
10.1016/0304-4165(91)90098-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
12-Lipoxygenase from rat basophilic leukemia cells was purified about 300-fold by protein-HPLC in a single run. Maximal 12-lipoxygenase activity was observed at pH 7.5, while the enzyme became almost inactive at pH 6 and 9. Although Ca2+ was not essential for 12-lipoxygenase activity, the partially purified enzyme was stimulated approx. 2-fold in the presence of 0.1-5.0 mM Ca2+. Contrary to 5-lipoxygenase from RBL-1 cells, 12-lipoxygenase was not inactivated by preincubation with Ca2+ for 1-10 min, nor was it stimulated by 0.1-10 mM ATP.
引用
收藏
页码：443 / 447
页数：5
相关论文
共 50 条
12345