CENTRIFUGAL COUNTERCURRENT CHROMATOGRAPHY, A PROMISING MEANS OF MEASURING PARTITION-COEFFICIENTS

Centrifugal counter-current chromatography (CCCC) was examined as a means of measuring lipophilicity values. Using an aqueous buffer as the stationary phase and 1-octanol as the eluent, seventeen solutes of widely different structure and of log Doct [log (distribution coefficient) at pH 7.41 spanning 2.5 units were examined. A very good correlation was found with literature log P [log (partition coefficient) of neutral species] values measured by the classical shake-flask method. The efficiency and precision of the CCCC method makes it a promising technique for measuring lipophilicity. © 1990.