IDENTIFICATION OF THE ACTIVE-SITE SERINE OF HORMONE-SENSITIVE LIPASE BY SITE-DIRECTED MUTAGENESIS

被引：56
|
作者：
HOLM, C
DAVIS, RC
OSTERLUND, T
SCHOTZ, MC
FREDRIKSON, G
机构：
[1] VET ADM WADSWORTH MED CTR,LOS ANGELES,CA
[2] UNIV CALIF LOS ANGELES,DEPT MED,LOS ANGELES,CA 90024
来源：
FEBS LETTERS | 1994年 / 344卷 / 2-3期
关键词：
LIPASE; ACTIVE SITE; SITE-DIRECTED MUTAGENESIS;
D O I：
10.1016/0014-5793(94)00403-X
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The consensus pentapeptide GXSXG is found in virtually all lipases/esterases and generally contains the active site serine. The primary sequence of hormone-sensitive lipase contains a single copy of this pentapeptide, surrounding Ser-423. We have analyzed the catalytic role of Ser-423 by site-directed mutagenesis and expression of the mutant hormone-sensitive lipase in COS cells. Substitution of Ser-423 by several different amino acids resulted in the complete abolition of both lipase and esterase activity, whereas mutation of other conserved serine residues had no effect on the catalytic activity. These results strongly suggest that Ser-423 is the active site serine of hormone-sensitive lipase.
引用
收藏
页码：234 / 238
页数：5
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