CLONING AND NUCLEOTIDE-SEQUENCE OF A FULL-LENGTH CDNA FOR HUMAN LIVER GAMMA-GLUTAMYLCYSTEINE SYNTHETASE

被引：126
|
作者：
GIPP, JJ [1 ]
CHANG, CS [1 ]
MULCAHY, RT [1 ]
机构：
[1] UNIV WISCONSIN,DEPT HUMAN ONCOL,MADISON,WI 53792
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 185卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/S0006-291X(05)80950-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have cloned and sequenced a full-length cDNA for human liver γ-glutamylcysteine synthetase (GCS), the rate-limiting enzyme in glutathione biosynthesis. The cDNA consists of 2634 bp containing an open reading frame encoding a protein of 367 amino acids and having a calculated Mr = 72,773. The nucleotide sequence of the cDNA for human liver GCS shares an 84% overall similarity with the composite rat GCS sequence deduced from three overlapping partial cDNAs (Yan and Meister, JBC 265: 1588-1593, 1990). The deduced amino acid sequences are 94% similar. Comparison of Northern blots of total RNA isolated from rat kidney or liver with that from human kidney revealed the GCS mRNA to be larger in the human tissue (∼4.0 kb vs. ∼3.7 kb). (The sequence for the human liver GCS cDNA has been assigned accession number M90656 in GenBank/EMBL databases.). © 1992 Academic Press, Inc.
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页码：29 / 35
页数：7
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