ISOPROTEIN ANALYSIS BY ION-EXCHANGE CHROMATOGRAPHY USING A LINEAR PH GRADIENT COMBINED WITH A SALT GRADIENT

被引：33
|
作者：
KALTENBRUNNER, O [1 ]
TAUER, C [1 ]
BRUNNER, J [1 ]
JUNGBAUER, A [1 ]
机构：
[1] UNIV WIEN,INST ANGEW MIKROBIOL,NUSSDORFER LANDE 11,A-1190 VIENNA,AUSTRIA
来源：
JOURNAL OF CHROMATOGRAPHY | 1993年 / 639卷 / 01期
关键词：
D O I：
10.1016/0021-9673(93)83086-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Isoproteins of human monoclonal antibodies with a pI range between 8.45 and 8.70 or 8.15 and 8.65 were separated by ion-exchange chromatography with a linear ascending pH gradient combined with a linear descending salt gradient using borax, mannitol and salt. The isoproteins were eluted according to their isoelectric points as demonstrated by conventional isoelectric focusing. Preparative purification and monitoring of the isoprotein composition of human monoclonal antibodies during a purification process is also presented to demonstrate the applicability of the method.
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页码：41 / 49
页数：9
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