CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF THE CAMP-DEPENDENT PROTEIN-KINASE CATALYTIC SUBUNIT FROM SACCHAROMYCES-CEREVISIAE

被引：10
|
作者：
KURET, J
PFLUGRATH, JW
机构：
[1] Cold Spring Harbor Laboratory, Cold Spring Harbor
来源：
BIOCHEMISTRY | 1991年 / 30卷 / 43期
关键词：
D O I：
10.1021/bi00107a031
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A truncated variant of TPK1, the yeast cAMP-dependent protein kinase catalytic subunit, was overexpressed in an engineered strain of Saccharomyces cerevisiae, purified by liquid chromatography, and crystallized from solutions of 2-propanol and magnesium at alkaline pH. The crystals are hexagonal dipyramids, space group P6(1)22 (P6(5)22), with unit-cell parameters a = b = 61 angstrom, c = 320 angstrom. Large single crystals suitable for diffraction analysis are obtainable by microseeding, and diffract beyond 2.8-angstrom resolution. Crystal density measurements reveal 12 kinase monomers per unit cell with a single kinase monomer per asymmetric unit.
引用
收藏
页码：10595 / 10600
页数：6
相关论文
共 50 条
  • [41] PHOSPHORYLATION OF PEPTIDE-SUBSTRATES FOR THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    ADAMS, JA
    TAYLOR, SS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1993, 268 (11) : 7747 - 7752
  • [42] EXPRESSION OF CDNAS FOR 2 ISOFORMS OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    UHLER, MD
    MCKNIGHT, GS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1987, 262 (31) : 15202 - 15207
  • [43] IDENTIFICATION OF PHOSPHORYLATION SITES IN THE RECOMBINANT CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE
    YONEMOTO, W
    GARROD, SM
    BELL, SM
    TAYLOR, SS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1993, 268 (25) : 18626 - 18632
  • [44] NUCLEOTIDE BINDING TO CHEMICALLY MODIFIED CATALYTIC SUBUNIT OF THE CAMP-DEPENDENT PROTEIN-KINASE
    BHATNAGAR, D
    HARTL, FT
    ROSKOSKI, R
    FEDERATION PROCEEDINGS, 1984, 43 (10) : 2471 - 2471
  • [45] EXPRESSION OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE IN ESCHERICHIA-COLI
    SLICE, LW
    TAYLOR, SS
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1989, 264 (35) : 20940 - 20946
  • [46] FACTORS THAT INFLUENCE THE NUCLEAR ACCESSIBILITY OF THE CATALYTIC SUBUNIT OF THE CAMP-DEPENDENT PROTEIN-KINASE
    WEN, W
    FANTOZZI, DA
    HAROOTUNIAN, AT
    FERAMISCO, JR
    TSIEN, RY
    MEINKOTH, JL
    TAYLOR, SS
    FASEB JOURNAL, 1994, 8 (07): : A1226 - A1226
  • [47] EXPRESSION OF THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE IN ESCHERICHIA-COLI
    SLICE, LW
    TAYLOR, SS
    FASEB JOURNAL, 1988, 2 (04): : A593 - A593
  • [48] AN UNUSUAL CATALYTIC SUBUNIT FOR THE CAMP-DEPENDENT PROTEIN-KINASE OF DICTYOSTELIUM-DISCOIDEUM
    ANJARD, C
    ETCHEBEHERE, L
    PINAUD, S
    VERON, M
    REYMOND, CD
    BIOCHEMISTRY, 1993, 32 (37) : 9532 - 9538
  • [49] HUMAN RECOMBINANT FACTOR-XIII FROM SACCHAROMYCES-CEREVISIAE - CRYSTALLIZATION AND PRELIMINARY-X-RAY DATA
    BISHOP, PD
    LASSER, GW
    LETRONG, I
    STENKAMP, RE
    TELLER, DC
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1990, 265 (23) : 13888 - 13889
  • [50] PHOSPHORYLATION OF MAMMALIAN MYOSIN KINASES BY THE CATALYTIC SUBUNIT OF CAMP-DEPENDENT PROTEIN-KINASE OR CGMP-DEPENDENT PROTEIN-KINASE
    NISHIKAWA, M
    DELANEROLLE, P
    LINCOLN, TM
    ADELSTEIN, RS
    BIOPHYSICAL JOURNAL, 1983, 41 (02) : A152 - A152
12345