INVESTIGATION OF METHODS OF ENZYME IMMOBILIZATION ON A COATED-WIRE ELECTRODE FOR THE DEVELOPMENT OF MICRO-BIOSENSORS

被引：26
|
作者：
TAGUCHI, H
ISHIHARA, N
OKUMURA, K
SHIMABAYASHI, Y
机构：
[1] Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, Tsu, Mie
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 228卷 / 01期
关键词：
D O I：
10.1016/S0003-2670(00)80492-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Tridodecylamine- and carboxyl-substituted poly(vinyl chloride)-based hydrogen ion-selective coated-wire electrodes are investigated as transducers for micro-biosensors. Various methods for immobilization of enzyme on the electrode were evaluated by using penicillinase as a model enzyme. The best method was immobilization with glutaraldehyde on the electrode previously modified with bovine serum albumin in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide to increase the amount of immobilized enzyme. A stable, large potential change is obtainable even in salt-containing samples with this sensor. The possibility of the practical use of such a micro-biosensor is suggested. © 1990.
引用
收藏
页码：159 / 162
页数：4
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