INVESTIGATION OF METHODS OF ENZYME IMMOBILIZATION ON A COATED-WIRE ELECTRODE FOR THE DEVELOPMENT OF MICRO-BIOSENSORS

Tridodecylamine- and carboxyl-substituted poly(vinyl chloride)-based hydrogen ion-selective coated-wire electrodes are investigated as transducers for micro-biosensors. Various methods for immobilization of enzyme on the electrode were evaluated by using penicillinase as a model enzyme. The best method was immobilization with glutaraldehyde on the electrode previously modified with bovine serum albumin in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide to increase the amount of immobilized enzyme. A stable, large potential change is obtainable even in salt-containing samples with this sensor. The possibility of the practical use of such a micro-biosensor is suggested. © 1990.