CLONING OF THE GENES ENCODING UREASE FROM PROTEUS-VULGARIS AND SEQUENCING OF THE STRUCTURAL GENES

被引：42
|
作者：
MORSDORF, G [1 ]
KALTWASSER, H [1 ]
机构：
[1] UNIV SAARLAND,FAC MIKROBIOL,IM STADTWALD,W-6600 SAARBRUCKEN,GERMANY
来源：
FEMS MICROBIOLOGY LETTERS | 1990年 / 66卷 / 1-3期
关键词：
Cloning; Nucleotide sequence; Proteus vulgaris; Urease;
D O I：
10.1016/0378-1097(90)90260-W
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A fragment of chromosomal DNA from Proteus vulgaris encoding urease was cloned and expressed in Escherchia coli. A 3 kbp region was sequenced and revealed three open reading frames with homology to jack (Canavalia ensiformis) urease. The smallest protein (11 kDa) was homologous to the N-terminus of the plant enzyme and the largest polypeptide (61 kDa) corresponded to the C-terminus. The large protein contained conserved regions and a cysteine residue which is known to be catalytically important in the plant enzyme. A protein of 12 kDa showed homology to residues 132 to 237 of jack bean urease. © 1989.
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收藏
页码：67 / 73
页数：7
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