THE SEGMENT-SPECIFIC GENE KROX-20 ENCODES A TRANSCRIPTION FACTOR WITH BINDING-SITES IN THE PROMOTER REGION OF THE HOX-1.4 GENE

被引:183
|
作者
CHAVRIER, P
VESQUE, C
GALLIOT, B
VIGNERON, M
DOLLE, P
DUBOULE, D
CHARNAY, P
机构
[1] ECOLE NORM SUPER,DEPT BIOL,CNRS,D1302,GENET MOLEC LAB,F-75230 PARIS 05,FRANCE
[2] FAC MED STRASBOURG,INSERM,U184,CNRS,GENET MOLEC EUCARYOTES LAB,F-67085 STRASBOURG,FRANCE
来源
EMBO JOURNAL | 1990年 / 9卷 / 04期
关键词
central nervous system; DNA-binding protein; homeobox; rhombomere; serum-inducible gene; transcription control; zinc finger;
D O I
10.1002/j.1460-2075.1990.tb08228.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Krox-20 is a mouse zinc finger gene expressed in a segment-specific manner in the early central nervous system, which makes it a potential developmental control gene. In this report, we show that the Krox-20 protein binds in vitro to two specific DNA sites located upstream from the homeobox containing gene Hox-1.4. The nucleotide sequence recognized by Krox-20 is closely related to the Sp1 target sequence, which is consistent with the similarity existing between the zinc fingers of the two proteins. In co-transfection experiments in cultured cells, Krox-20 dramatically activates transcription from the herpes simplex virus thymidine kinase promoter when an oligomer of its binding site is present in cis close to the promoter. Analysis of mutated binding sites demonstrated that the level of activation by Krox-20 correlates with the affinity of the protein for the mutant sequence. These data indicate that Krox-20 constitutes a sequence-specific DNA-binding transcription factor. Parallel analysis of the expression of Krox-20 and Hox-1.4 in the neural tube by in situ hybridization revealed no overlap, arguing against direct interactions between these two genes. The possible involvement of Krox-20 in the regulation of the transcription of other homeobox genes is discussed in view of their respective patterns of expression.
引用
收藏
页码:1209 / 1218
页数:10
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