PURIFICATION OF HUMAN LIVER FUMARYLACETOACETASE USING IMMUNOAFFINITY CHROMATOGRAPHY

被引：2
|
作者：
VANFAASSEN, H [1 ]
VANDENBERG, IET [1 ]
BERGER, R [1 ]
机构：
[1] UNIV GRONINGEN,DEPT PEDIAT,10 BLOEMSINGEL,9712 KZ GRONINGEN,NETHERLANDS
来源：
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 20卷 / 04期
关键词：
Fumarylacetoacetase; Hydrolase; Immunoaffinity chromatography; Protein/enzyme purification; Tyrosine; Tyrosinemia type I;
D O I：
10.1016/0165-022X(90)90093-R
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described to purify fumarylacetoacetase from crude human liver extracts using immunoaffinity chromatography. Immobilized rabbit antibodies specific for beef liver fumarylacetoacetase were used as an immunoadsorbent. With this rapid and specific procedure human liver fumarylacetoacetase could be purified to apparent homogeneity. The molecular weight of native human liver fumarylacetoacetase is approximately 83000 as estimated by gel filtration. The two subunits have a molecular weight of approximately 41000, as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Purified human liver fumarylacetoacetase has a broad pH optimum with a maximum at pH 7.2 and a Km = 2.1 μM towards fumarylacetoacetate. © 1990.
引用
收藏
页码：317 / 324
页数：8
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