GALACTOSE-BINDING SITE IN ESCHERICHIA-COLI HEAT-LABILE ENTEROTOXIN (LT) AND CHOLERA-TOXIN (CT)

The galactose-binding site in cholera toxin and the closely related heat-labile enterotoxin (LT) from Escherichia coli is an attractive target for the rational design of potential anti-cholera drugs. In this paper we analyse the molecular structure of this binding site as seen in several crystal structures, including that of an LT:galactose complex which we report here at 2.2 Angstrom resolution. The binding surface on the free toxin contains several tightly associated water molecules and a relatively flexible loop consisting of residues 51-60 of the B subunit. During receptor binding this loop becomes tightly ordered by forming hydrogen bonds jointly to the G(M1) pentasaccharide and to a set of water molecules which stabilize the toxin:receptor complex.