ISOLATION AND INACTIVATION OF THE NUCLEAR GENE ENCODING THE ROTENONE-INSENSITIVE INTERNAL NADH - UBIQUINONE OXIDOREDUCTASE OF MITOCHONDRIA FROM SACCHAROMYCES-CEREVISIAE

被引：105

作者：

MARRES, CAM

DEVRIES, S

GRIVELL, LA

机构：

[1] UNIV AMSTERDAM,DEPT MOLEC CELL BIOL,MOLEC BIOL SECT,KRUISLAAN 318,1098 SM AMSTERDAM,NETHERLANDS

[2] UNIV AMSTERDAM,CTR BIOTECHNOL,1098 SM AMSTERDAM,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1991年 / 195卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1991.tb15775.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have recntly described the isolation of a mitochondrial rotenone-insensitive NADH:ubiquinone oxidoreductase from Saccharomyces cerevisiae [de Vries, S. & Grivell, L. A. (1988) Eur. J. Biochem. 176, 377-384]. We now report the isolation of the nuclear gene encoding this single-subunit enzyme. Null mutants have been constructed by means of one-step gene disruption. Oxygen-uptake experiments, performed with mitochondria isolated from the mutant cells, showed that this NADH dehydrogenase catalyzes the oxidation of NADH generated inside the mitochondrion. Inactivation of this NADH dehydrogenase does not affect growth on glucose and ethanol, but growth on lactate, pyruvate and acetate is impaired or absent. This phenotype is discussed in terms of the interplay between different metabolic pathways in yeast.

引用

页码：857 / 862

页数：6

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