AUTOMATED-DETERMINATION OF AMINO-ACIDS IN SEAWATER

被引：5
|
作者：
WENCK, A
MINTROP, L
DUINKER, JC
机构：
[1] Institut fuer Meereskunde, Abt. Meereschemie, W-2300 Kiel
来源：
MARINE CHEMISTRY | 1991年 / 33卷 / 1-2期
关键词：
D O I：
10.1016/0304-4203(91)90053-Y
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel reaction buffer for the derivatization of amino acids in seawater with the o-phthaldialdehyde/2-mercaptoethanol reagent is introduced. This buffer involves citric instead of boric acid and effectively prevents the precipitation of Ca and Mg as hydroxides or carbonates under the alkaline conditions required for derivatization. With this buffer, an autosampler with the capability of pre-column derivatization can be used for the HPLC-separation and fluorimetric detection of dissolved free amino acids in seawater.
引用
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页码：1 / 7
页数：7
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