Evaluation of FISH for Blood Cultures under Diagnostic Real-Life Conditions

被引:3
|
作者
Reitz, Annalena [1 ]
Poppert, Sven [2 ,3 ]
Rieker, Melanie [4 ]
Frickmann, Hagen [5 ,6 ]
机构
[1] Goethe Univ, Univ Hosp, Frankfurt, Germany
[2] Swiss Trop & Publ Hlth Inst, Basel, Switzerland
[3] Univ Basel, Fac Med, Basel, Switzerland
[4] MVZ Humangenet Ulm, Ulm, Germany
[5] Bundeswehr Hosp Hamburg, Dept Microbiol & Hosp Hyg, Bernhard Nocht Str 74, D-20359 Hamburg, Germany
[6] Univ Hosp Rostock, Inst Med Microbiol Virol & Hyg, Rostock, Germany
来源
EUROPEAN JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY | 2018年 / 8卷 / 04期
关键词
fluorescence in-situ hybridization; blood culture; rapid diagnostics; molecular diagnostics; sepsis;
D O I
10.1556/1886.2018.00024
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: The study assessed a spectrum of previously published in-house fluorescence in-situ hybridization (FISH) probes in a combined approach regarding their diagnostic performance with incubated blood culture materials. Methods: Within a two-year interval, positive blood culture materials were assessed with Gram and FISH staining. Previously described and new FISH probes were combined to panels for Gram-positive cocci in grape-like clusters and in chains, as well as for Gram-negative rod-shaped bacteria. Covered pathogens comprised Staphylococcus spp., such as S. aureus, Micrococcus spp., Enterococcus spp., including E. faecium, E. faecalis, and E. gallinarum, Streptococcus spp., like S. pyogenes, S. agalactiae, and S. pneumoniae, Enterobacteriaceae, such as Escherichia coli, Klebsiella pneumoniae and Salmonella spp., Pseudomonas aeruginosa, Stenotrophomonas maltophilia, and Bacteroides spp. Results: A total of 955 blood culture materials were assessed with FISH. In 21 (2.2%) instances, FISH reaction led to non-interpretable results. With few exemptions, the tested FISH probes showed acceptable test characteristics even in the routine setting, with a sensitivity ranging from 28.6% (Bacteroides spp.) to 100% (6 probes) and a specificity of >95% in all instances. Conclusion: If sophisticated rapid diagnostic methods like mass spectrometry from blood culture materials are not available, FISH provides an option for rapid differentiation for laboratories in resource-limited settings.
引用
收藏
页码:135 / 141
页数:7
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