DETERMINATION OF 6-KETO-PGF1-ALPHA, 2,3-DINOR-6-KETO-PGF1-ALPHA, THROMBOXANE-B2, 2,3-DINOR-THROMBOXANE-B2, PGE2, PGD2 AND PGF2-ALPHA IN HUMAN URINE BY GAS-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL IONIZATION MASS-SPECTROMETRY

被引：21

作者：

WEBER, C ^{[1
]}

HOLLER, M ^{[1
]}

BEETENS, J ^{[1
]}

DECLERCK, F ^{[1
]}

TEGTMEIER, F ^{[1
]}

机构：

[1] JANSSEN RES FDN, B-2340 BEERSE, BELGIUM

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 562卷 / 1-2期

关键词：

D O I：

10.1016/0378-4347(91)80611-F

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method for quantification of 6-keto-PGF1-alpha, TXB2, 2,3-dinor TXB2, PGE2, PGD2 and PGF2-alpha in human urine samples, using gas chromatography-negative ion chemical ionization mass spectrometry, is described. Deuterated analogues were used as internal standards. Methoximation was carried out in urine samples which were subsequently applied to phenylboronic acid cartridges, reversed-phase cartridges and thin-layer chromatography. The eluents were further derivatized to pentafluorobenzyl ester trimethylsilyl ethers for final quantification by gas chromatography-mass spectrometry. The overall recovery was 77% for tritiated 6-keto-PGF1alpha and 55% for tritiated TXB2. Urinary levels of prostanoids were determined in a group of six volunteers before and after intake of the thromboxane synthase inhibitor Ridogrel, and related to creatinine clearance.

引用

页码：599 / 611

页数：13

共 50 条

[31] PROSTAGLANDINS - PGF2-ALPHA, PGE2, 6-KETO-PGF1-ALPHA AND TXB2 SERUM LEVELS IN DYSMENORRHEIC ADOLESCENTS BEFORE, DURING AND AFTER TREATMENT WITH ORAL-CONTRACEPTIVES
CREATSAS, G
DELIGEOROGLOU, E
ZACHARI, A
LOUTRADIS, D
PAPADIMITRIOU, T
MIRAS, K
ARAVANTINOS, D
EUROPEAN JOURNAL OF OBSTETRICS & GYNECOLOGY AND REPRODUCTIVE BIOLOGY, 1990, 36 (03) : 292 - 298
[32] EFFECTS OF SECRETIN ON PGE2 AND 6-KETO-PGF1-ALPHA LEVELS IN HUMAN GASTRIC-MUCOSA
MINE, T
FUJISAKI, J
AKIMOTO, K
YOSHIDA, S
OHSAWA, H
HASEGAWA, Y
OGATA, E
DIGESTIVE DISEASES AND SCIENCES, 1986, 31 (10) : S446 - S446
[33] FACILE METHOD FOR PREPARATION OF 2,3-DINOR-6-KETO PGF1-ALPHA THE MAJOR URINARY METABOLITE OF PROSTACYCLIN
BALAZY, M
BRASS, EP
GERBER, JG
NIES, AS
PROSTAGLANDINS, 1988, 36 (04): : 421 - 430
[34] URINARY CONCENTRATIONS OF PGE-2, PGF-2-ALPHA, 6-KETO-PGF-1-ALPHA AND TXB-2 IN CHILDREN
FASCE, L
PATRONO, C
CORDONE, G
CIABATTONI, G
GIANFRANCESCHI, P
RIVISTA ITALIANA DI PEDIATRIA-ITALIAN JOURNAL OF PEDIATRICS, 1981, 7 (04): : 431 - 432
[35] A STUDY OF PLASMA-LEVELS OF THE ARACHIDONIC-ACID DERIVATIVES PGE2, PGF2-ALPHA, TXB2 AND 6-KETO-PGF1-ALPHA IN SURGICAL PATIENTS IN INTENSIVE-CARE
KESSLER, P
ALEXANDRITIS, T
SCHWULERA, U
KLEIN, G
LISSNER, R
ANAESTHESIST, 1988, 37 (11): : 720 - 721
[36] REBOUND ELEVATION IN URINARY THROMBOXANE-B2 AND 6-KETO-PGF1-ALPHA-EXCRETION AFTER ASPIRIN WITHDRAWAL
VIAL, JH
MCLEOD, LJ
ROBERTS, MS
ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH, 1991, 21 : 157 - 160
[37] SIMPLIFIED ASSAY FOR THE QUANTIFICATION OF 2,3-DINOR-6-KETO-PROSTAGLANDIN-F(1-ALPHA) BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
DANIEL, VC
MINTON, TA
BROWN, NJ
NADEAU, JH
MORROW, JD
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1994, 653 (02): : 117 - 122
[38] Urinary excretion of 2,3-dinor-6-keto-PGF(1 alpha) and 11-dehydro-TXB(2) by the gravid spontaneously hypertensive rat
Schaefer, WR
Seufert, RJ
Casper, FW
Zahradnik, HP
PROSTAGLANDINS, 1996, 52 (01): : 1 - 11
[39] THE EFFECT OF LABETALOL ON PLATELET CONSUMPTION 6-KETO PGF-1-ALPHA AND 6-KETO PGF-1-ALPHA-THROMBOXANE-B2 RATIO IN PRE-ECLAMPSIA
WALKER, JJ
GREER, IA
MCLAREN, M
LANG, GD
BELCH, JJF
FORBES, CD
PRENTICE, CRM
CALDER, AA
BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 1983, 90 (11): : 1094 - 1094
[40] GAS CHROMATOGRAPHY MASS SPECTROMETRY MEASUREMENT OF PGE2 PGF2ALPHA, 13, 14 DIHYDRO-15 KETO-PGE2, AND 13, 14 DIHYDRO-15 KETO-PGF2ALPHA
MAYNARD, S
SAVORY, J
CLINICAL CHEMISTRY, 1977, 23 (06) : 1123 - 1123

← 1 2 3 4 5 →