GROWTH-INHIBITORY EFFECTS OF 1,25-DIHYDROXYVITAMIN-D3 ON NORMAL HUMAN KERATINOCYTES CULTURED IN SERUM-FREE MEDIUM

被引：118
|
作者：
MATSUMOTO, K
HASHIMOTO, K
NISHIDA, Y
HASHIRO, M
YOSHIKAWA, K
机构：
[1] Department of Dermatology, Osaka University School of Medicine, Fukushima, Fukushima-ku, Osaka
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 166卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90898-W
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on the growth of normal human keratinocytes cultured in serum-free medium was investigated. 1,25(OH)2D3 inhibited the cell growth at 10-7 M by 75.3% and at 10-6 M almost completely. The growth inhibition was accompanied by changes related to proliferation: (1) remarkable inhibition of DNA synthesis, (2) the decrease in the number of high-affinity receptors for epidermal growth factor, with almost no change in total receptor number, (3) the rapid decrease in c-myc mRNA level. The inhibition of DNA synthesis and the decrease of c-myc mRNA expression occurred at 3 h after the addition of 1,25(OH)2D3. These results suggest that decrease of c-myc mRNA expression is one of the primary effects of 1,25(OH)2D3 in the growth inhibition of human keratinocytes. © 1990.
引用
收藏
页码：916 / 923
页数：8
相关论文
共 50 条
  • [41] INHIBITION OF HUMAN CANCER CELL-GROWTH BY 1,25-DIHYDROXYVITAMIN-D3 METABOLITES
    FRAMPTON, RJ
    OMOND, SA
    EISMAN, JA
    CANCER RESEARCH, 1983, 43 (09) : 4443 - 4447
  • [42] 1,25-DIHYDROXYVITAMIN-D3 RAPIDLY ALTERS THE MORPHOLOGY OF THE DUODENAL MUCOSA OF RACHITIC CHICKS - EVIDENCE FOR NOVEL EFFECTS OF 1,25-DIHYDROXYVITAMIN-D3
    MCCARTHY, JT
    BARHAM, SS
    KUMAR, R
    JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1984, 21 (03): : 253 - 258
  • [43] FREE 1,25-DIHYDROXYVITAMIN-D CONSTITUTES A SMALL FRACTION OF TOTAL 1,25-DIHYDROXYVITAMIN-D IN NORMAL HUMAN-SERUM
    SMAGULA, C
    AKRIGHT, LS
    ADAMS, ND
    DUNN, JF
    CLINICAL RESEARCH, 1983, 31 (05): : A883 - A883
  • [44] BINDING AND METABOLISM OF 1,25-DIHYDROXYVITAMIN-D3 IN CULTURED BOVINE PARATHYROID CELLS
    BROWN, AJ
    BERKOBEN, M
    RITTER, CS
    SLATOPOLSKY, E
    ENDOCRINOLOGY, 1992, 130 (01) : 276 - 281
  • [45] EVIDENCE FOR 1,25-DIHYDROXYVITAMIN-D3 PRODUCTION BY CULTURED PORCINE ALVEOLAR MACROPHAGES
    REICHEL, H
    BISHOP, JE
    KOEFFLER, HP
    NORMAN, AW
    MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1991, 75 (02) : 163 - 167
  • [46] ONLY 1,25-DIHYDROXYVITAMIN-D3 IS NEEDED FOR NORMAL BONE-GROWTH AND MINERALIZATION IN THE RAT
    MATHEWS, CHE
    PARFITT, AM
    BROMMAGE, R
    JARNAGIN, K
    DELUCA, HF
    CALCIFIED TISSUE INTERNATIONAL, 1983, 35 (4-5) : 699 - 699
  • [47] SEX STEROID AND 1,25-DIHYDROXYVITAMIN-D3 RECEPTORS IN HUMAN COLORECTAL ADENOCARCINOMA AND NORMAL MUCOSA
    MEGGOUH, F
    LOINTIER, P
    SAEZ, S
    CANCER RESEARCH, 1991, 51 (04) : 1227 - 1233
  • [48] RESISTANCE TO 1,25-DIHYDROXYVITAMIN-D3 OF CULTURED PSORIATIC EPIDERMAL-KERATINOCYTES ISOLATED FROM INVOLVED AND UNINVOLVED SKIN
    ABE, J
    KONDO, S
    NISHII, Y
    KUROKI, T
    JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1989, 68 (04): : 851 - 854
  • [49] THE EFFECT OF 1,25-DIHYDROXYVITAMIN-D3 ON CARTILAGE GROWTH IN NEONATAL MICE
    SILBERMANN, M
    MIRSKY, N
    LEVITAN, S
    WEISMAN, Y
    METABOLIC BONE DISEASE & RELATED RESEARCH, 1983, 4 (06): : 337 - 345
  • [50] INTERACTION OF ANDROGEN AND 1,25-DIHYDROXYVITAMIN-D3 - EFFECTS ON NORMAL RAT BONE-CELLS
    GRAY, C
    COLSTON, KW
    MACKAY, AG
    TAYLOR, ML
    ARNETT, TR
    JOURNAL OF BONE AND MINERAL RESEARCH, 1992, 7 (01) : 41 - 46
12345