REPRODUCIBLE ACQUISITION OF ESCHERICHIA-COLI PORIN SURFACE TOPOGRAPHS BY ATOMIC-FORCE MICROSCOPY

被引：108

作者：

SCHABERT, FA ^{[1
]}

ENGEL, A ^{[1
]}

机构：

[1] UNIV BASEL,BIOZENTRUM,MAURICE E MULLER INST MICROSCOP STRUCT BIOL,CH-4056 BASEL,SWITZERLAND

来源：

BIOPHYSICAL JOURNAL | 1994年 / 67卷 / 06期

关键词：

D O I：

10.1016/S0006-3495(94)80726-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Crystalline membranes reconstituted from Escherichia coli OmpF porin and phospholipids were adsorbed to freshly cleaved mica and imaged in solution by the atomic force microscope. The extracellular as well as the periplasmic side of the porin trimers could be identified and the conditions to record topographs at 1-nm lateral and 0.1-nm vertical resolution were established.

引用

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页码：2394 / 2403

页数：10

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