Investigation the Single Nucleotide Polymorphism of KISS 1 gene Associated with Pubertal of Iraq Goat

In this study, we have reviewed progress regarding the relationship between kiss peptin and puberty. This study was conducted on 25 Iraqi goat (prepubertal and pubertal) provide from January 2016 to January 2017 age of animals ranged from 4 month to the one years. Blood samples from jugular vein (5 ml) with anticoagulant to extraction DNA by intron-kit for PCR technique to estimated the size of band of Kiss-1 gene to PCR-RFLP to limited genotype of Iraqi goat for differentiated between prepubertal and pubertaland these analysis was doi ng in biotechnology department / Al-Nahrain University. The results in this study was homozygous CC377bp,TT with fragments digested at 256 and 121 bp and TC377, 256 and 121 bpgenotype of intron 1 of KISS1 geneas well as single nucleotide polymorphismT/C in located 98 and the Finallythe result show high significant in FSH and estrogen hormone when compared prepubertal Iraqi Goat with pubertal(P<0.0001). In conclusion of this study RFLP-PCR of KISS 1prepubertalIraqi Goat was highly significant P<0.01 compared with pubertal.