NICOTINIC RECEPTOR-MEDIATED RELEASE OF NORADRENALINE IN THE HUMAN NEUROBLASTOMA SH-SY5Y

被引：49

作者：

VAUGHAN, PFT ^{[1
]}

KAYE, DF ^{[1
]}

REEVE, HL ^{[1
]}

BALL, SG ^{[1
]}

PEERS, C ^{[1
]}

机构：

[1] UNIV LEEDS, DEPT PHARMACOL, LEEDS LS2 9JT, W YORKSHIRE, ENGLAND

来源：

JOURNAL OF NEUROCHEMISTRY | 1993年 / 60卷 / 06期

关键词：

NORADRENALINE RELEASE; NICOTINIC RECEPTORS; SHSY5Y NEUROBLASTOMA; DIMETHYLPHENYLPIPERAZINIUM IODIDE; L-TYPE CALCIUM CHANNELS;

D O I：

10.1111/j.1471-4159.1993.tb03501.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Dimethylphenylpiperazinium iodide (a nicotinic agonist) evokes noradrenaline release from human neuroblastoma SH-SY5Y cells that have been pretreated with 12-O-tetradecanoylphorbol 13-acetate for 8 min. This effect of dimethylphenylpiperazinium iodide was inhibited by 1 muM mecamylamine but not by 1 muM atropine, which suggests that SH-SY5Y cells express nicotinic receptors coupled to the release of noradrenaline. Dimethylphenylpiperazinium iodide-evoked release was enhanced by 5 muM Bay K 8644 (an L-type calcium agonist) and inhibited by 1 muM nifedipine. Dimethylphenylpiperazinium iodide depolarised SH-SY5Y cells and enhanced the level of intracellular calcium in cells loaded with fura 2. The effects of dimethylphenylpiperazinium iodide on noradrenaline release, depolarisation, and intracellular calcium levels were all inhibited by 1 muM desmethylimipramine. The results of this study show that nicotinic receptors in SH-SY5Y cells stimulate noradrenaline release by activation of L-type calcium channels.

引用

页码：2159 / 2166

页数：8

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