A CONVENIENT CLONING VECTOR CONTAINING THE GAL4 DNA-BINDING DOMAIN

被引：3

作者：

RAYCROFT, L ^{[1
]}

LOZANO, G ^{[1
]}

机构：

[1] UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC GENET,1515 HOLCOMBE BLVD,BOX 11,HOUSTON,TX 77030

来源：

GENE | 1992年 / 118卷 / 01期

关键词：

RECOMBINANT DNA; FUSION PROTEINS; TRANSACTIVATION;

D O I：

10.1016/0378-1119(92)90262-N

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A DNA fragment encoding the yeast GAL4 DNA-binding domain (amino acids 3-147) was cloned into a convenient vector. This vector contains unique restriction sites at both the 5' and 3' ends and allows the generation of fusion proteins containing the GAL4 DNA-binding domain. These fusion proteins can be tested for their ability to activate transcription.

引用

页码：143 / 144

页数：2

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