LIMITED REPERTOIRE OF THE C-TERMINAL REGION OF THE M-PROTEIN IN STREPTOCOCCUS-PYOGENES

被引：12

作者：

RELF, WA ^{[1
]}

SRIPRAKASH, KS ^{[1
]}

机构：

[1] MENZIES SCH HLTH RES,POB 41096,CASUARINA 0811,AUSTRALIA

来源：

FEMS MICROBIOLOGY LETTERS | 1990年 / 71卷 / 03期

关键词：

M protein; Opacity factor; Polymerase chain reaction; Streptococcus pyogenes;

D O I：

10.1016/0378-1097(90)90245-L

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have amplified genomic sequences (emm) that may encode M protein from strains of Streptococcus pyogenes using the polymerase chain reaction (PCR). Genomic DNA from 22 isolates representing 14 M scrotypes was selected for the study. Primers which corresponded to the observed C-terminal sequences of emm6, emm49 and ennX were used. PCR products using emm6 and emm49 oligonucleotides were classified into two mutually exclusive groups which corresponded to the presence or absence of serum opacity factor. These findings support the concept of limited heterogeneity in the C-terminal sequences of the M protein. © 1990.

引用

页码：345 / 350

页数：6

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