LIPID ANALYSIS AND FATTY-ACID PROFILES OF INDIVIDUAL ARTERIAL ATHEROSCLEROTIC PLAQUES

被引：22
|
作者：
MCGRATH, LT [1 ]
ELLIOTT, RJ [1 ]
机构：
[1] QUEENS UNIV BELFAST, SCH BIOL & BIOCHEM, DIV BIOCHEM, BELFAST BT9 7BL, ANTRIM, NORTH IRELAND
来源：
ANALYTICAL BIOCHEMISTRY | 1990年 / 187卷 / 02期
关键词：
D O I：
10.1016/0003-2697(90)90456-J
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A protocol for the analysis of the lipid profile of microsamples of aortic tissue was developed. Lipid extraction was from intact tissue using acetone and chloroform/methanol ( 2 1, v/v). The extract was analyzed for total lipid, esterified cholesterol, cholesterol, triacylglycerol, and phospholipid. The extract was then processed to separate cholesteryl esters, triacylglycerol, and phospholipid which were hydrolyzed and the fatty acid composition was determined by GLC of pentaflu-orobenzyl ester derivatives. A lipid profile could be obtained on samples with a wet weight of 5 mg. © 1990.
引用
收藏
页码：273 / 276
页数：4
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