GAS-CHROMATOGRAPHY MASS-SPECTROMETRY ANALYSIS OF 2-AMINO-1-METHYL-6-PHENYL-IMIDAZO[4,5-B]PYRIDINE IN URINE AND FECES

被引：14
|
作者：
FRIESEN, MD [1 ]
GARREN, L [1 ]
BEREZIAT, JC [1 ]
KADLUBAR, F [1 ]
LIN, DX [1 ]
机构：
[1] NATL CTR TOXICOL RES,JEFFERSON,AR 72079
来源：
ENVIRONMENTAL HEALTH PERSPECTIVES | 1993年 / 99卷
关键词：
D O I：
10.2307/3431476
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
A method has been developed to measure levels of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) excreted in urine and feces. The method involves organic solvent extraction, derivatization to form electron-capturing bis-pentafluorobenzyl derivatives, and analysis by gas chromatography-negative ion chemical ionization mass spectrometry using a deuterium- labeled internal standard. The method can detect PhIP at levels of less than 1 ng/g in rat urine (5 ng/24 hr) and 5 ng/g (wet weight) in rat feces (50 ng/24 hr). Sprague-Dawley rats given a single 50 mug dose of PhIP by gavage excreted an average of 0.6% of the dose in the urine and 25% of the dose in the feces as unchanged PhIP, in the first 4 days after treatment. To make this method applicable for the analyses of biological fluids of PhIP-exposed human subjects, it is now being improved by using immunoaffinity chromatography.
引用
收藏
页码：179 / 181
页数：3
相关论文
共 50 条
  • [31] METABOLISM OF 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE (PHIP) IN MICE
    BUONARATI, MH
    ROPER, M
    MORRIS, CJ
    HAPPE, JA
    KNIZE, MG
    FELTON, JS
    CARCINOGENESIS, 1992, 13 (04) : 621 - 627
  • [32] Synthesis of a new isomer of creatinine and its use in the preparation of the food mutagen 2-amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine (PHIP)
    Bergman, Jan
    TETRAHEDRON LETTERS, 2009, 50 (40) : 5631 - 5632
  • [33] SYNTHESIS OF 2-AMINO-SUBSTITUTED OF 3-METHYL-3H-IMIDAZO 4,5-B!PYRIDINE
    VUTILOV, YM
    BYSTROVA, RM
    KHIMIYA GETEROTSIKLICHESKIKH SOEDINENII, 1970, (08): : 1146 - &
  • [34] Biomonitoring the cooked meat carcinogen 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine in hair and prostate by ultra performance liquid chromatography-mass spectrometry
    Xiao, Shun
    Krishnamachari, Sesha
    Weight, Christopher
    Turesky, Robert
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2016, 252
  • [35] 4-(2-AMINO-1-METHYLIMIDAZO[4,5-B]PYRID-6-YL)PHENYL SULFATE - A MAJOR METABOLITE OF THE FOOD MUTAGEN 2-AMINO-1-METHYL-6-PHENYLIMIDAZO[4,5-B]PYRIDINE (PHIP) IN THE RAT
    ALEXANDER, J
    WALLIN, H
    HOLME, JA
    BECHER, G
    CARCINOGENESIS, 1989, 10 (08) : 1543 - 1547
  • [36] Synthesis and decomposition of an ester derivative of the procarcinogen and promutagen, PhIP, 2-amino-1-methyl-6-phenyl-1H-imidazo[4,5-b]pyridine:: Unusual nitrenium ion chemistry
    Nguyen, Thach-Mien
    Novak, Michael
    JOURNAL OF ORGANIC CHEMISTRY, 2007, 72 (13): : 4698 - 4706
  • [37] ANALYSIS OF METHYLATED AND OXIDIZED PURINES IN URINE BY CAPILLARY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY
    STILLWELL, WG
    XU, HX
    ADKINS, JA
    WISHNOK, JS
    TANNENBAUM, SR
    CHEMICAL RESEARCH IN TOXICOLOGY, 1989, 2 (02) : 94 - 99
  • [38] MECHANISM OF INTERFERENCES FOR GAS-CHROMATOGRAPHY MASS-SPECTROMETRY ANALYSIS OF URINE FOR DRUGS OF ABUSE
    WU, AHB
    ANNALS OF CLINICAL AND LABORATORY SCIENCE, 1995, 25 (04): : 319 - 329
  • [39] Active secretion of 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine (PhIP) in the colon
    von Keutz, A.
    Schroeder, B.
    Breves, G.
    Steinberg, P.
    Nicken, P.
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2012, 385 : 63 - 63
  • [40] Comparison of crystal structure and theory for 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine
    Parkin, S
    Marsch, GA
    Hope, H
    Whitney, E
    Winter, NW
    Colvin, ME
    Felton, JS
    Turteltaub, KW
    CHEMICAL RESEARCH IN TOXICOLOGY, 1996, 9 (03) : 574 - 579
12345