CELLULAR AND TISSUE DISTRIBUTION OF A SINGLE-STRAND-SPECIFIC NUCLEASE

被引：1

作者：

VAVATSI, NA ^{[1
]}

KOUIDOU, SA ^{[1
]}

TRIANTOS, AA ^{[1
]}

KAVOUKOPOULOS, EM ^{[1
]}

PAPAGEORGIOU, GE ^{[1
]}

TRAKATELLIS, AC ^{[1
]}

机构：

[1] ARISTOTELIAN UNIV SALONIKA,SCH MED,DEPT BIOL CHEM,GR-54006 SALONIKA,GREECE

来源：

INTERNATIONAL JOURNAL OF BIOCHEMISTRY | 1991年 / 23卷 / 01期

关键词：

D O I：

10.1016/0020-711X(91)90005-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

1. Specific antibodies which were raised against a single-strand- specific nuclease isolated from rat liver microsomes were used for characterizing this enzyme and determining its cellular and tissue distribution. 2. The single-strand-specific nuclease does not show any homology with other known nucleolytic enzymes. 3. It is mainly localized in microsomes and cytosol; traces of it are also found in nuclei, but it could not be detected in mitochondria. 4. Using the same specific antibodies we attempted to detect this nuclease in some other tissues which exhibit high metabolic rates, namely kidneys, heart and spleen. 5. Thus, with the aid of immunological techniques we were able to determine that at least part of the total poly(U) nucleolytic activity observed in kidney and heart is due to a nuclease immunologically identical to the enzyme under study. Kidneys were the best source for this enzyme.

引用

页码：27 / 31

页数：5

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