The effects of storage temperature and time on the microflora of fresh, vacuum packaged, and modified atmospheres packaged asparagus (Asparagus officinalis) were analyzed. Total aerobic mesophilic, psychrophilic, lactic acid bacteria, Enterobacteriaceae, and yeast and molds were determined as a function of time. The effect of washing on the original microflora of the asparagus and the growth of the different microbial groups during the storage time were checked. From day 8, a progressive increase in the mesophile and psychrophile counts in both types of packaging was observed. At the end of the experiment (day 21), the mesophile and psychrophile counts were all 10(7) CFU/g, for both types of packaging. In the vacuum packaging, the final enterobacteria counts (2.5 x 10(2) CFU/g) and yeast and molds (10 CFU/g) were lower than in the polyethylene bag packaged asparagus, which showed enterobacteria counts of 7.3 x 10(4) CFU/g and yeast and mold counts of 2.3 x 10(4) CFU/g. The non-packaged asparagus showed low counts from the fourth day due to their heavy dehydration caused by the low relative humidity in the storage chamber.