X-ALPHA-GAL-BASED MEDIUM FOR SIMULTANEOUS ENUMERATION OF BIFIDOBACTERIA AND LACTIC-ACID BACTERIA IN MILK

被引：29

作者：

CHEVALIER, P ^{[1
]}

ROY, D ^{[1
]}

SAVOIE, L ^{[1
]}

机构：

[1] AGR CANADA,CTR FOOD RES & DEV,LACT ACID BACTERIA RES GRP,3600 CASAVANT BLVD W,ST HYACINTHE J2S 8E3,QUEBEC,CANADA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 1991年 / 13卷 / 01期

关键词：

BIFIDOBACTERIUM; DIFFERENTIAL ENUMERATION; X-ALPHA-GAL; ALPHA-GALACTOSIDASE; PERMEABILIZATION PROCEDURE; PRESUMPTIVE IDENTIFICATION;

D O I：

10.1016/0167-7012(91)90034-N

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method using the chromogenic substrate 5-bromo-4-chloro-3-indolyl-alpha-D-galactoside was developed for the differential enumeration of Bifidobacterium spp. and lactic acid bacteria used in preparation of fermented and nonfermented dairy products. The methodology is based on the high alpha-galactosidase activity of bifidobacteria as compared to that of Lactobacillus or Streptococcus strains. The X-alpha-Gal-based medium is useful to identify bifidobacteria among Lactobacillus since the enzyme action of alpha-galactosidase splits X-alpha-Gal substrate and releases indol which imparts a blue colour to bifidobacterial colonies on agar plates. The blue colonies can also be assigned to the genus Bifidobacterium using a simple permeabilization procedure with Triton X-100 which allows the reliable determination of fructose-6-phosphate phosphoketolase activity.

引用

页码：75 / 83

页数：9

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