ENANTIOSEPARATION OF 5-MONOSUBSTITUTED HYDANTOINS BY CAPILLARY GAS-CHROMATOGRAPHY - INVESTIGATION OF CHEMICAL AND ENZYMATIC RACEMIZATION

被引：11
|
作者：
LICKEFETT, H
KROHN, K
KONIG, WA
GEHRCKE, B
SYLDATK, C
机构：
[1] Institut f. Biochemie u. Biotechnologie der Technischen Universität Btannschweig Konstantin, W-3300 Braunschweig
[2] Fachbereich Chemie der Universitäts-GH-Paderborn, W-4790 Paderborn
[3] Institut f. organische Chemie der Universität Hamburg, W-2000 Hamburg 13
来源：
TETRAHEDRON-ASYMMETRY | 1993年 / 4卷 / 06期
关键词：
D O I：
10.1016/S0957-4166(00)80219-0
中图分类号：
O61 [无机化学];
学科分类号：
070301 ; 081704 ;
摘要：
The chemical as well as the enzymatic racemization of R- and S-5-monosubstituted hydantoin derivatives play an important role in the chemoenzymatic synthesis of optically pure D- or L-amino acids. To study these reactions, specifically with 5-alkylhydantoins enantiomer-separation by capillary gas chromatography, octakis(2,6-di-O-methyl-3-O-pentyl)-gamma-cyclodextrin as a chiral stationary phase was used. It will be shown that this method allows the sensitive and reproducible detection of a wide range of substrates in a short time. Examples are given for the chemical racemization of different 5-alkylhydantoins as well as for their enzymatic racemization by a hydantoin racemase of Arthrobacter sp. DSM 3745.
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页码：1129 / 1135
页数：7
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