TRANSCRIPTIONAL REPRESSION MEDIATED BY THE WT1 WILMS-TUMOR GENE-PRODUCT

被引：492
|
作者：
MADDEN, SL
COOK, DM
MORRIS, JF
GASHLER, A
SUKHATME, VP
RAUSCHER, FJ
机构：
[1] WISTAR INST, 3601 SPRUCE ST, PHILADELPHIA, PA 19104 USA
[2] HOWARD HUGHES MED INST, DEPT MED MOLEC GENET & CELL BIOL, CHICAGO, IL 60637 USA
来源：
SCIENCE | 1991年 / 253卷 / 5027期
关键词：
D O I：
10.1126/science.1654597
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The wt1 gene, a putative tumor suppressor gene located at the Wilms tumor (WT) locus on chromosome 11p13, encodes a zinc finger-containing protein that binds to the same DNA sequence as EGR-1, a mitogen-inducible immediate-early gene product that activates transcription. The transcriptional regulatory potential of WT1 has not been demonstrated. In transient transfection assays, the WT1 protein functioned as a repressor of transcription when bound to the EGR-1 site. The repression function was mapped to the glutamine- and proline-rich NH2-terminus of WT1; fusion of this domain to the zinc finger region of EGR-1 converted EGR-1 into a transcriptional repressor.
引用
收藏
页码：1550 / 1553
页数：4
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