PREPARATION AND USE OF A MONOCLONAL-ANTIBODY TO DETECT CHLAMYDIA-PSITTACI ANTIGEN IN PARAFFIN-EMBEDDED TISSUE-SECTIONS

被引：3
|
作者：
BOLLO, E
BIOLATTI, B
DONN, A
TURILLI, C
WILSMORE, AJ
机构：
[1] ROYAL VET COLL,DEPT ANIM HLTH,POTTERS BAR EN6 1NB,HERTS,ENGLAND
[2] IST ZOOPROFILATT SPERIMENTALE VENEZIE,I-35100 PADUA,ITALY
来源：
RESEARCH IN VETERINARY SCIENCE | 1992年 / 53卷 / 03期
关键词：
D O I：
10.1016/0034-5288(92)90146-S
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A murine monoclonal antibody prepared against an ovine abortion isolate of Chlamydia psittaci (A22/Teramo) revealed specific binding to a 57 kDa chlamydial antigen in immunoblotting studies. The monoclonal antibody was able to detect intracytoplasmic chlamydial inclusions and scattered elementary bodies in infected McCoy cell culture, and on formalin-fixed paraffin-embedded tissue sections both from experimentally infected mice and from fetal membranes of cases of ovine enzootic abortion.
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页码：393 / 396
页数：4
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