SEQUENCE ORGANIZATION OF THE HUMAN-CHROMOSOME 2Q TELOMERE

被引：53
|
作者：
MACINA, RA
NEGOREV, DG
SPAIS, C
RUTHIG, LA
HU, XL
RIETHMAN, HC
机构：
[1] WISTAR INST ANAT & BIOL,PHILADELPHIA,PA 19104
[2] RUSSIAN ACAD SCI,INST MOLEC GENET,MOSCOW 123182,RUSSIA
来源：
HUMAN MOLECULAR GENETICS | 1994年 / 3卷 / 10期
关键词：
D O I：
10.1093/hmg/3.10.1847
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The terminal 240 kb of a human 2q telomere region was cloned in two overlapping yeast artificial-chromosomes (YACs). This DNA contains a region of low-copy subtelomeric repeats (within 50 kb of the 2q telomere), a segment of DNA duplicated on distal 8p23 (100 kb from the 2q telomere), and a region of single-copy DNA (230 kb from the 2q telomere). Two CpG islands are present in the DNA segment duplicated on distal 8p23. RecA-assisted restriction endonuclease cleavage of genomic DNA samples revealed a potential 55 kb chromosome length polymorphism at the 2q telomere. This work provides telomeric closure of maps for human chromosome 2q, demonstrates a novel, subtelomere-specific DNA duplication, and will permit detailed molecular and cytological studies of this human telomere region.
引用
收藏
页码：1847 / 1853
页数：7
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