3D SUPER-RESOLUTION FLUORESCENCE MICROSCOPY USING CYLINDRICAL VECTOR BEAMS

被引：5

作者：

Suyama, Taikei ^{[1
]}

Zhang, Yaoju ^{[2
]}

机构：

[1] Akashi Natl Coll Technol, Dept Elect & Comp Engn, Akashi, Hyogo 6748501, Japan

[2] Wenzhou Univ, Coll Phys & Elece Informat, Wenzhou 325035, Peoples R China

来源：

PROGRESS IN ELECTROMAGNETICS RESEARCH LETTERS | 2013年 / 43卷 / 43期

基金：

中国国家自然科学基金;

关键词：

Bright spots - Cylindrical vector beam - Double ring - Radially polarized beam - Super resolution - Three-dimensional resolution - Truncation parameter - Vector-vortex beams;

D O I：

10.2528/PIERL13080205

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We propose a method to obtain nano-scale 3D super resolution in STED fluorescence microscopy. A double-ring-shaped cylindrical vector vortex beam, with an appropriate vortex angle and a proper truncation parameter of the beam, is used to generate a 3D dark spot as the erase spot. A single-ring-shaped radially polarized beam is used as a pump beam, which can generate a sharper 3D bright spot. The volume of the generated 3D dark spot is small and the uniformity of the light wall surrounding the spot is quite high. Consequently, the 3D super-resolution ability of a STED microscope is improved and nano-scale three-dimensional resolutions are obtained.

引用

页码：73 / 81

页数：9

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