CYTOGENETIC STUDIES ON HUMAN LIVER-CANCER CELL-LINES

被引：20
|
作者：
CHEN, HL
CHIU, TS
CHEN, PJ
CHEN, DS
机构：
[1] NATL TAIWAN UNIV HOSP,HEPATITIS RES CTR,7 CHUNG SHAN S RD,TAIPEI 100,TAIWAN
[2] NATL TAIWAN UNIV HOSP,GRAD INST CLIN MED,TAIPEI 100,TAIWAN
[3] COLL MED,TAIPEI,TAIWAN
来源：
CANCER GENETICS AND CYTOGENETICS | 1993年 / 65卷 / 02期
关键词：
D O I：
10.1016/0165-4608(93)90227-D
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Karyotyping of human liver cancer cell lines and chromosome in situ hybridization of hepatitis B virus (HBV) DNA was performed in order to elucidate the possible mechanism of hepatocarcinogenesis from evidence of chromosomal changes and HBV integration patterns. HepG2-2 and HepG2-5 cell lines were HepG2 cells experimentally transfected with HBV DNA; the HCC36 cell line was derived from a hepatocellular carcinoma containing integrated HBV DNA from a Taiwanese patient. HepG2 cells, a hepatoblastoma cell line without HBV DNA integration, served as negative control. In HepG2-2, HepG2-5, and HCC36 cells, multiple integrations of HBV DNA were observed by in situ hybridization and hybridization signals occurred preferentially on certain chromosomes: 2, 5, 10, 11, 18; 7, 10, 13, 17, 18; and 4, 6, 11, 12q+, 18; respectively. In addition, a strong correlation between chromosomal changes and HBV integration was noticed in HCC36 cells, especially at chromosome 12q+.
引用
收藏
页码：161 / 166
页数：6
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