TRANSFORMATION OF ACETOBACTER POLYOXOGENES WITH PLASMID DNA BY ELECTROPORATION

被引：1

作者：

TAYAMA, K ^{[1
]}

FUKAYA, M ^{[1
]}

OKUMURA, H ^{[1
]}

KAWAMURA, Y ^{[1
]}

HORINOUCHI, S ^{[1
]}

BEPPU, T ^{[1
]}

机构：

[1] UNIV TOKYO, FAC AGR, DEPT AGR CHEM, BUNKYO KU, TOKYO 113, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1994年 / 58卷 / 05期

关键词：

D O I：

10.1271/bbb.58.974

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Acetobacter polyoxogenes was transformed with plasmid DNA by electroporation. The following points were essential for transformation: (i) dilution of the culture broth with cold water and air bubbling of the culture broth for transformation at discharging from a jar fermenter, and (ii) selection of transformants by liquid cultivation. For shortening of the lag time in cultivation for selection of transformants, the following treatments were useful: (i) addition of sucrose to the cell suspension during transformation and to the broth for cultivation, and (ii) addition of 1 mM MgCl2 to a mixture of cells and DNA during electroporation.

引用

页码：974 / 975

页数：2

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