STIMULATION OF AGROBACTERIUM-TUMEFACIENS T-DNA TRANSFER BY OVERDRIVE DEPENDS ON A FLANKING SEQUENCE BUT NOT ON HELICAL POSITION WITH RESPECT TO THE BORDER REPEAT

被引：17

作者：

SHURVINTON, CE ^{[1
]}

REAM, W ^{[1
]}

机构：

[1] OREGON STATE UNIV, DEPT AGR CHEM, PROGRAM MOLEC & CELLULAR BIOL, CORVALLIS, OR 97331 USA

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 17期

关键词：

D O I：

10.1128/jb.173.17.5558-5563.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.

引用

页码：5558 / 5563

页数：6

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