STIMULATION OF AGROBACTERIUM-TUMEFACIENS T-DNA TRANSFER BY OVERDRIVE DEPENDS ON A FLANKING SEQUENCE BUT NOT ON HELICAL POSITION WITH RESPECT TO THE BORDER REPEAT

被引:17
|
作者
SHURVINTON, CE [1 ]
REAM, W [1 ]
机构
[1] OREGON STATE UNIV, DEPT AGR CHEM, PROGRAM MOLEC & CELLULAR BIOL, CORVALLIS, OR 97331 USA
关键词
D O I
10.1128/jb.173.17.5558-5563.1991
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
T-DNA transfer by Agrobacterium tumefaciens depends on the right border repeat of the T-DNA and is greatly stimulated by overdrive, an adjacent sequence. We report that the function of overdrive does not depend on helical position with respect to the border repeat. A synthetic 24-bp overdrive and a 12-bp region containing a fully conserved 8-bp core overdrive sequence stimulated virulence equally, but full function required additional bases to the left of the 24-bp sequence.
引用
收藏
页码:5558 / 5563
页数:6
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