Effects of the combination of vitamin K and teriparatide on the bone metabolism in ovariectomized rats

被引:24
|
作者
Nagura, Nana [1 ]
Komatsu, Jun [1 ]
Iwase, Hideaki [2 ]
Hosoda, Hiroshi [3 ]
Ohbayashi, Osamu [1 ]
Nagaoka, Isao [3 ]
Kaneko, Kazuo [1 ]
机构
[1] Juntendo Univ, Grad Sch Med, Dept Med Motor Organs, Tokyo 1138421, Japan
[2] Juntendo Univ, Inst Casualty Ctr, Dept Bioengn, Shizuoka 4102295, Japan
[3] Juntendo Univ, Grad Sch Med, Dept Host Def & Biochem Res, 2-1-1 Hongo, Tokyo 1138421, Japan
关键词
vitamin K; teriparatide; bone metabolism; osteocalcin; ovariectomized rats;
D O I
10.3892/br.2015.431
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
The purpose of the present study was to evaluate the combined effects of vitamin K (VK) and teriparatide (TPTD) on bone mineral density (BMD), mechanical strength and other parameters for bone metabolism using a rat ovariectomized osteoporosis model. Ovariectomized female Sprague-Dawley rats were administered with VK (an oral dose of 30 mg/kg/day), TPTD (a subcutaneous dose of 30 mu g/kg, three times a week) or a combination for 8 weeks. Thereafter, serum levels of gamma-carboxylated osteocalcin (Gla-OC) were quantitated by ELISA; BMD and mechanical strength were measured by computed tomography and biomechanical testing, respectively at the femoral metaphysis. Additionally, histo-morphometry was performed using the toluidine blue-stained coronal sections of distal femur. The combination of VK and TPTD clearly increased the serum levels of Gla-OC (a specific marker for bone formation) and osteoblast surface (the number of osteoblasts attaching with the surface of cancellous bone), compared to VK or TPTD alone. In addition, the combination of the two agents improved the BMD and bone strength of the femur in the ovariectomized rats, compared to VK or TPTD alone. Taken together, these findings suggest that the treatment with VK and TPTD may have a therapeutic advantage over VK or TPTD monotherapy for postmenopausal osteoporosis, possibly by enhancing the bone formation through the actions on OC and osteoblasts.
引用
收藏
页码:295 / 300
页数:6
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