INTRACELLULAR PH MEASURED BY P-31-NMR IN ISOLATED RAT-HEART DURING ALPHA-1-ADRENOCEPTOR STIMULATION

被引：2
|
作者：
HANEM, S
GRONAS, T
HOLTEN, T
JYNGE, P
SKOMEDAL, T
OSNES, JB
机构：
[1] UNIV OSLO, DEPT PHARMACOL, N-0316 OSLO, NORWAY
[2] UNIV TRONDHEIM, FAC MED, DEPT PHARMACOL & TOXICOL, N-7030 TRONDHEIM, NORWAY
来源：
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY | 1994年 / 26卷 / 05期
关键词：
PH; ALPHA-1-ADRENERGIC STIMULATION; NMR;
D O I：
10.1006/jmcc.1994.1079
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Changes of intracellular pH (pHi) after alpha-1-adrenoceptor stimulation (5 × 10−5 mol/phenylephrine in the presence of 10−6 mol/l timolol) were studied in isolated perfused rat hearts by 31 P-NMR spectroscopy in order to use the whole organ without disruption. The contractility was monitored simultaneously in all hearts. Alpha-1-adrenoceptor stimulation gave an alkalinization by 0.02 ± 0.01 pH-units (mean ± S.E.M.) after 5 min and by 0.03 ± 0.01 pH-units (mean ± S.E.M.) after 15 min. Thus, an intracellular alkalinization after myocardial alpha-1-adrenoceptor stimulation was observed during close to physiological conditions. The elevation of pHi may contribute to maintain the inotropic effect alpha-1-adrenoceptor stimulation. © 1994 Academic Press Limited.
引用
收藏
页码：669 / 674
页数：6
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