PURIFICATION OF EQUINE NEUTROPHIL LYSOZYME AND ITS ANTIBACTERIAL ACTIVITY AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA

被引：12
|
作者：
PELLEGRINI, A
WAIBLINGER, S
VONFELLENBERG, R
机构：
[1] Division of Applied Veterinary Physiology, Institute of Veterinary Physiology, University of Zürich, Zürich, CH-8057
来源：
VETERINARY RESEARCH COMMUNICATIONS | 1991年 / 15卷 / 06期
关键词：
BACTERIA; CHROMATOGRAPHY; HORSE; LYSOZYME; NEUTROPHILS;
D O I：
10.1007/BF00346538
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Lysozyme from equine neutrophil granulocytes was isolated in a pure from by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. The lysozyme lysed Micrococcus luteus, Bacillus subtilis and Staphylococcus lentus and was also bactericidal against the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Bordetella bronchiseptica, and Serratia marcescens. Staphylococcus aureus and Staphylococcus epidermidis were not lysed. The lysozyme was only very slightly bactericidal for S. epidermidis and and S. aureus. Equine neutrophil lysozyme was found to be bactericidal for Gram-positive as well as for Gram-negative bacteria without further treatment. Equine and chicken egg white lysozymes were found to be immunologically related when examined using specific antisera against each of them. Both lysozymes also had very similar specific enzymatic activities against M. luteus membranes.
引用
收藏
页码：427 / 435
页数：9
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