ISOLATION OF ISLETS OF LANGERHANS FROM THE WEANLING PIG

被引：0
|
作者：
HEALD, KA
HAIL, CA
DOWNING, R
机构：
来源：
DIABETES RESEARCH CLINICAL AND EXPERIMENTAL | 1991年 / 17卷 / 01期
关键词：
ISLETS OF LANGERHANS; PIG PANCREAS; PERCOLL; COLLAGENASE; ISOLATION;
D O I：
暂无
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A simple method for the isolation of porcine islets of Langerhans is described. The technique. based on sequential collagenase digestion and density gradient separation through Percoll at unit gravity, produces islets which secrete insulin in response to glucose stimulation in static incubation, show bi-phasic release of insulin when perifused with 16mM glucose, and can be maintained in culture for six weeks.
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页码：7 / 12
页数：6
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