ENDOSOMAL PROTEOLYSIS PRECEDES RICIN A-CHAIN TOXICITY IN MACROPHAGES

被引：22
|
作者：
FIANI, ML [1 ]
BLUM, JS [1 ]
STAHL, PD [1 ]
机构：
[1] WASHINGTON UNIV,SCH MED,DEPT CELL BIOL & PHYSIOL,ST LOUIS,MO 63110
来源：
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS | 1993年 / 307卷 / 02期
关键词：
D O I：
10.1006/abbi.1993.1583
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Ricin A-chain is delivered into macrophages via receptor-mediated endocytosis. We have found that following uptake via the mannose receptor, ricin A-chain is rapidly cleaved by endosomal proteases. Inhibition of endosomal protenses such as cathepsin D and B leads to the accumulation of toxin inside the cell. Inhibition of cathepsin D reduces ricin A-chain cytotoxicity, while blocking cathepsin B enhances cytotoxicity. Similar results were obtained using fibroblasts transfected with the mannose receptor. Our data strongly suggest that the activation or membrane translocation of ricin A-chain is dependent upon the action of specific proteases. © 1993 Academic Press, Inc.
引用
收藏
页码：225 / 230
页数：6
相关论文
共 50 条
  • [31] The N-terminal ricin propeptide influences the fate of ricin A-chain in tobacco protoplasts
    Jolliffe, Nicholas A.
    Di Cola, Alessandra
    Marsden, Catherine J.
    Lord, J. Michael
    Ceriotti, Aldo
    Frigerio, Lorenzo
    Roberts, Lynne M.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2006, 281 (33) : 23377 - 23385
  • [32] Reductive activation of ricin and ricin A-chain immunotoxins by protein disulfide isomerase and thioredoxin reductase
    Bellisola, G
    Fracasso, G
    Ippoliti, R
    Menestrina, G
    Rosén, A
    Soldá, S
    Udali, S
    Tomazzolli, R
    Tridente, G
    Colombatti, M
    BIOCHEMICAL PHARMACOLOGY, 2004, 67 (09) : 1721 - 1731
  • [33] Vinyldeoxyadenosine in a sarcin-ricin RNA loop and its binding to ricin toxin A-chain
    Roday, Setu
    Saen-oon, Suwipa
    Schramm, Vern L.
    BIOCHEMISTRY, 2007, 46 (21) : 6169 - 6182
  • [34] BREFELDIN-A ENHANCEMENT OF RICIN A-CHAIN IMMUNOTOXINS AND BLOCKADE OF INTACT RICIN, MODECCIN, AND ABRIN
    HUDSON, TH
    GRILLO, FG
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1991, 266 (28) : 18586 - 18592
  • [35] Recognition and interaction of small rings with the ricin A-chain binding site
    Yan, XJ
    Day, P
    Hollis, T
    Monzingo, AF
    Schelp, E
    Robertus, JD
    Milne, GWA
    Wang, SM
    PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1998, 31 (01): : 33 - 41
  • [36] In vitro selection of RNA molecules that inhibit the activity of ricin A-chain
    Hesselberth, JR
    Miller, D
    Robertus, J
    Ellington, AD
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (07) : 4937 - 4942
  • [37] Poly(amidoamine)-mediated intracytoplasmic delivery of ricin A-chain and gelonin
    Pattrick, NG
    Richardson, SCW
    Casolaro, M
    Ferruti, P
    Duncan, R
    JOURNAL OF CONTROLLED RELEASE, 2001, 77 (03) : 225 - 232
  • [38] ANTIBODY TO ONCOGENE PROTEIN PRODUCT AND ITS CONJUGATE WITH RICIN A-CHAIN
    刘辉
    隋文作
    刘连瑞
    Chinese Journal of Cancer Research, 1991, (04) : 51 - 54
  • [39] SENSITIVITY OF MALIGNANT LYMPHOID-CELLS TO RICIN A-CHAIN IMMUNOTOXINS
    LAURENT, G
    CASELLAS, P
    DEROCQ, JM
    PONCELET, P
    DUSSOSSOY, D
    JANSEN, F
    BRITISH JOURNAL OF CANCER, 1985, 52 (04) : 652 - 652
  • [40] Is there a clinical value for ricin A-chain immunotoxins in Hodgkins lymphoma.
    Schnell, R
    Staak, JO
    Vitetta, E
    Schindler, J
    Borchmann, P
    Barth, S
    Diehl, V
    Engert, A
    BLOOD, 2000, 96 (11) : 248B - 248B
12345