CHARACTERIZATION OF HUMAN BRONCHIAL EPITHELIAL-CELLS IMMORTALIZED BY THE E6 AND E7 GENES OF HUMAN PAPILLOMAVIRUS TYPE-16

被引：48
|
作者：
VIALLET, J
LIU, C
EMOND, J
TSAO, MS
机构：
[1] MONTREAL GEN HOSP & RES INST, DEPT MED & ONCOL, MONTREAL, PQ, CANADA
[2] MONTREAL GEN HOSP & RES INST, DEPT PATHOL, MONTREAL, PQ, CANADA
[3] MCGILL UNIV, MONTREAL, PQ, CANADA
来源：
EXPERIMENTAL CELL RESEARCH | 1994年 / 212卷 / 01期
关键词：
D O I：
10.1006/excr.1994.1115
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be more sensitive to terminal differentiation induced by phorbol esters than the parental line, but both are refractory to the effects of serum. HBE4-E6/E7 cells are able to form tubules when grown in a basement membrane-like matrix, indicating that the cellular pathways leading to immortalization and those governing morphogenesis are distinct. This cell line should prove useful in the study of bronchial epithelial cell growth, differentiation, morphogenesis, and carcinogenesis. (C) 1994 Academic Press, Inc.
引用
收藏
页码：36 / 41
页数：6
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