CHARACTERIZATION OF HUMAN BRONCHIAL EPITHELIAL-CELLS IMMORTALIZED BY THE E6 AND E7 GENES OF HUMAN PAPILLOMAVIRUS TYPE-16

被引：48
|
作者：
VIALLET, J
LIU, C
EMOND, J
TSAO, MS
机构：
[1] MONTREAL GEN HOSP & RES INST, DEPT MED & ONCOL, MONTREAL, PQ, CANADA
[2] MONTREAL GEN HOSP & RES INST, DEPT PATHOL, MONTREAL, PQ, CANADA
[3] MCGILL UNIV, MONTREAL, PQ, CANADA
来源：
EXPERIMENTAL CELL RESEARCH | 1994年 / 212卷 / 01期
关键词：
D O I：
10.1006/excr.1994.1115
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be more sensitive to terminal differentiation induced by phorbol esters than the parental line, but both are refractory to the effects of serum. HBE4-E6/E7 cells are able to form tubules when grown in a basement membrane-like matrix, indicating that the cellular pathways leading to immortalization and those governing morphogenesis are distinct. This cell line should prove useful in the study of bronchial epithelial cell growth, differentiation, morphogenesis, and carcinogenesis. (C) 1994 Academic Press, Inc.
引用
收藏
页码：36 / 41
页数：6
相关论文
共 50 条
  • [1] CALCIUM REGULATES THE DIFFERENTIATION OF HUMAN PAPILLOMAVIRUS TYPE-16 (HPV16) IMMORTALIZED ECTOCERVICAL EPITHELIAL-CELLS, BUT NOT THE EXPRESSION OF THE PAPILLOMAVIRUS E6 AND E7 ONCOGENES
    CHOO, CK
    RORKE, EA
    ECKERT, RL
    [J]. EXPERIMENTAL CELL RESEARCH, 1993, 208 (01) : 161 - 169
  • [2] METASTATIC CONVERSION OF CELLS BY EXPRESSION OF HUMAN PAPILLOMAVIRUS TYPE-16 E6 AND E7 GENES
    CHEN, LP
    ASHE, S
    SINGHAL, MC
    GALLOWAY, DA
    HELLSTROM, I
    HELLSTROM, KE
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (14) : 6523 - 6527
  • [3] EXPRESSION OF CYTOCHROME-P450 AND MICROSOMAL EPOXIDE HYDROLASE IN CERVICAL AND ORAL EPITHELIAL-CELLS IMMORTALIZED BY HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 GENES
    FARIN, FM
    BIGLER, LG
    ODA, D
    MCDOUGALL, JK
    OMIECINSKI, CJ
    [J]. CARCINOGENESIS, 1995, 16 (06) : 1391 - 1401
  • [4] THE E7 GENE OF HUMAN PAPILLOMAVIRUS TYPE-16 IS SUFFICIENT FOR IMMORTALIZATION OF HUMAN EPITHELIAL-CELLS
    HALBERT, CL
    DEMERS, GW
    GALLOWAY, DA
    [J]. JOURNAL OF VIROLOGY, 1991, 65 (01) : 473 - 478
  • [5] Establishment and Characterization of Immortalized Human Dermal Papilla Cells Expressing Human Papillomavirus 16 E6/E7
    Kim, Seonhwa
    Jeon, Kyeong-Bae
    Park, Hyo-Min
    Kim, Jinju
    Lim, Chae-Min
    Yoon, Do -Young
    [J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2024, 34 (03) : 506 - 515
  • [6] INDUCTION OF UTERINE CERVICAL NEOPLASIAS IN MICE BY HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 GENES
    SASAGAWA, T
    INOUE, M
    INOUE, H
    YUTSUDO, M
    TANIZAWA, O
    HAKURA, A
    [J]. CANCER RESEARCH, 1992, 52 (16) : 4420 - 4426
  • [7] IN-VITRO AND IN-VIVO INHIBITION OF HUMAN PAPILLOMAVIRUS TYPE-16 E6 AND E7 GENES
    TAN, TMC
    TING, RCY
    [J]. CANCER RESEARCH, 1995, 55 (20) : 4599 - 4605
  • [8] EXPRESSION OF CYTOCHROME-P450 AND MICROSOMAL EXPOSIDE HYDROLASE IN CERVICAL AND ORAL EPITHELIAL-CELLS IMMORTALIZED BY HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 GENES (VOL 16, PG 1391, 1995)
    FARIN, FM
    BIGLER, LG
    ODA, D
    MCDOUGALL, JK
    OMIECINSKI, CJ
    [J]. CARCINOGENESIS, 1995, 16 (07) : 1670 - 1670
  • [9] NEUROEPITHELIAL CARCINOMAS IN MICE TRANSGENIC WITH HUMAN PAPILLOMAVIRUS TYPE-16 E6/E7 ORFS
    ARBEIT, JM
    MUNGER, K
    HOWLEY, PM
    HANAHAN, D
    [J]. AMERICAN JOURNAL OF PATHOLOGY, 1993, 142 (04): : 1187 - 1197
  • [10] Genomic instability and telomerase activity in human bronchial epithelial cells during immortalization by human papillomavirus-16 E6 and E7 genes
    Coursen, JD
    Bennett, WP
    Gollahon, L
    Shay, JW
    Harris, CC
    [J]. EXPERIMENTAL CELL RESEARCH, 1997, 235 (01) : 245 - 253
12345