CALORIMETRIC CHARACTERIZATION OF THE STABLE COMPLEX OF MYOSIN SUBFRAGMENT 1 WITH ADP AND BERYLLIUM FLUORIDE

被引：30
|
作者：
BOBKOV, AA
KHVOROV, NV
GOLITSINA, NL
LEVITSKY, DI
机构：
[1] A N BACH BIOCHEM INST,MOSCOW 117071,RUSSIA
[2] MOSCOW MV LOMONOSOV STATE UNIV,A N BELOZERSKY INST PHYS CHEM BIOL,MOSCOW 119899,RUSSIA
来源：
FEBS LETTERS | 1993年 / 332卷 / 1-2期
关键词：
MYOSIN SUBFRAGMENT 1; BERYLLIUM FLUORIDE; SCANNING MICROCALORIMETRY; RABBIT SKELETAL MUSCLE;
D O I：
10.1016/0014-5793(93)80485-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The thermal unfolding of the myosin subfragment 1 (S1) in its stable complex with ADP and beryllium fluoride (S1 . ADP . BeF3-) was studied by differential scanning calorimetry. It has been shown that the structure of the S1 molecule in the S1 . ADP . BeF3- complex is similar to that of S1 in its complex with ADP and orthovanadate (S1 . ADP . V(i)) but differs radically from that of nucleotide-free S1 and S1 in the S1 . ADP complex. It is concluded that the S1 . ADP . BeF3- complex can be considered, like the S1 . ADP . V(i) complex, a stable structural analogue of the myosin head . ADP . P(i) transition state of the myosin-catalyzed ATP hydrolysis.
引用
收藏
页码：64 / 66
页数：3
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