THE DIFFERENCE BETWEEN ADP-BERYLLIUM FLUORIDE AND ADP-ALUMINUM FLUORIDE COMPLEXES OF THE SPIN-LABELED MYOSIN SUBFRAGMENT-1

被引：33
|
作者：
PONOMAREV, MA
TIMOFEEV, VP
LEVITSKY, DI
机构：
[1] RUSSIAN ACAD SCI,BACH INST BIOCHEM,MOSCOW 117071,RUSSIA
[2] MOSCOW MV LOMONOSOV STATE UNIV,SCH BIOL,DEPT BIOCHEM,MOSCOW 119899,RUSSIA
[3] RUSSIAN ACAD SCI,ENGELHARDT INST MOLEC BIOL,MOSCOW 117984,RUSSIA
来源：
FEBS LETTERS | 1995年 / 371卷 / 03期
关键词：
MYOSIN SUBFRAGMENT 1; SPIN-LABELING; ALUMINUM FLUORIDE; BERYLLIUM FLUORIDE;
D O I：
10.1016/0014-5793(95)00898-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Electron paramagnetic resonance (EPR) spectroscopy was used for investigation of the structure of spin-labeled myosin subfragment 1 (S1) containing ADP and phosphate analogues, such as orthovanadate, aluminium fluoride (AIF(4)), and beryllium fluoride (BeFx). It has been shown that the local conformational changes in the region of Cys-707, induced by formation of the S1-ADP-BeFx complex, differ from those of S1 containing ADP-AIF(4) or other phosphate analogues but are similar to the changes which occur in the presence of ADP or ATP gamma S, It is suggested that S1-ADP-AIF(4) and S1-ADP-BeFx complexes represent structural analogues of different transition states of the ATPase cycle, namely the intermediate states S1**-ADP-P-1 and S1*-ATP, respectively.
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页码：261 / 263
页数：3
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