MYOINOSITOL TRISPHOSPHATE MOBILIZES CALCIUM FROM FUSOGENIC CARROT (DAUCUS-CAROTA L) PROTOPLASTS

被引：49
|
作者：
RINCON, M [1 ]
BOSS, WF [1 ]
机构：
[1] N CAROLINA STATE UNIV, DEPT BOT, RALEIGH, NC 27695 USA
来源：
PLANT PHYSIOLOGY | 1987年 / 83卷 / 02期
关键词：
D O I：
10.1104/pp.83.2.395
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
To determine whether or not inositol trisphosphate (IP3) mobilizes calcium in higher plant cells, we investigated the effect of IP3 on Ca2+ fluxes in fusogenic carrot (Daucus carota L.) protoplasts. The protoplasts were incubated in 45Ca2+-containing medium and the 45Ca2+ associated with the protoplasts was monitored with time. Addition of IP3 (20 micromolar) caused a 17% net loss of the accumulated 45Ca2+ within 4 minutes. There was a reuptake of 45Ca2+ and the protoplasts recovered to their initial value by 10 minutes. Phytic acid (IP6), also stimulated 45Ca2+ efflux from the protoplasts. Both the IP3- and the IP6-induced 45Ca2+ efflux were inhibited by the calmodulin antagonist, trifluoperazine.
引用
收藏
页码：395 / 398
页数：4
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