CLONING AND ANALYSIS OF A CDNA-ENCODING A HUMAN LIVER CARBOXYLESTERASE

被引：24
|
作者：
RIDDLES, PW [1 ]
RICHARDS, LJ [1 ]
BOWLES, MR [1 ]
POND, SM [1 ]
机构：
[1] UNIV QUEENSLAND,PRINCESS ALEXANDRA HOSP,DEPT MED,BRISBANE,QLD 4102,AUSTRALIA
来源：
GENE | 1991年 / 108卷 / 02期
关键词：
OLIGODEOXYRIBONUCLEOTIDES; ACTIVE SITE SERINE; PRIMARY STRUCTURE; MESSENGER RNA; RECOMBINANT DNA;
D O I：
10.1016/0378-1119(91)90448-K
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
A human liver carboxylesterase (CE)-encoding cDNA has been cloned using synthetic oligodeoxyribonucleotides (oligos) based on the known amino acid (aa) sequences of rabbit and rat liver CEs. The oligos hybridize specifically to DNA encoding liver CEs. The longest cDNA obtained from screening several cDNA libraries encodes about 80% of the protein and translates into an aa sequence which has a high degree of similarity with the sequences of liver CEs from other species. On hybridization to mRNA isolated from human liver, the cDNA gave a single band of about 2.0 kb consistent with its encoding a protein of < 68 kDa. DNA obtained from a number of human livers and probed with the CE cDNA gave identical hybridization patterns. These patterns were moderately complex by comparison with published data.
引用
收藏
页码：289 / 292
页数：4
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